Gene Expression and Regulation · ⁨Ekspresi dan Regulasi Gen⁩

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.1.A: Describe the structures involved in passing hereditary information from one generation to the next.

• 6.1.A.1 Genetic information is stored in and passed to subsequent generations through DNA molecules and, in some cases, RNA molecules.
  • 6.1.A.1.i Prokaryotic organisms typically have circular chromosomes.
  • 6.1.A.1.ii Eukaryotic organisms typically have multiple linear chromosomes that are comprised of DNA. These chromosomes are condensed using histones and associated proteins.
• 6.1.A.2 Prokaryotes and eukaryotes can contain plasmids, which are extra-chromosomal circular molecules of DNA.

Learning Objective 6.1.B: Describe the characteristics of DNA that allow it to be used as hereditary material.

• 6.1.B.1 Nucleic acids exhibit specific nucleotide base pairing that is conserved through evolution.
  • 6.1.B.1.i Purines (guanine and adenine) have a double ring structure.
  • 6.1.B.1.ii Pyrimidines (cytosine, thymine, and uracil) have a single ring structure.
  • 6.1.B.1.iii Purines pair with pyrimidines: adenine with thymine (or uracil in RNA) and guanine with cytosine.

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.2.A: Describe the mechanisms by which genetic information is copied for transmission between generations.

• 6.2.A.1 DNA replication ensures continuity of hereditary information.
  • 6.2.A.1.i DNA is synthesized in the 5' to 3' direction.
  • 6.2.A.1.ii Replication is a semiconservative process, meaning one strand of DNA serves as the template for a new strand of complementary DNA.
  • 6.2.A.1.iii Helicase unwinds the DNA strands.
  • 6.2.A.1.iv Topoisomerase relaxes supercoiling in front of the replication fork.
  • 6.2.A.1.v DNA polymerase requires RNA primers to initiate DNA synthesis.
  • 6.2.A.1.vi DNA polymerase synthesizes new strands of DNA continuously on the leading strand and discontinuously on the lagging strand.
  • 6.2.A.1.vii Ligase joins the fragments on the lagging strand.
  • Exclusion statement: The names of the steps and particular enzymes involved, excluding DNA polymerase, ligase, RNA polymerase, helicase, and topoisomerase, are beyond the scope of the AP Exam.

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.3.A: Describe the mechanisms by which genetic information flows from DNA to RNA to protein.

• 6.3.A.1 The sequence of the RNA bases, together with the structure of the RNA molecule, determines RNA function.
  • 6.3.A.1.i Messenger RNA (mRNA) molecules carry information from DNA in the nucleus to the ribosome in the cytoplasm.
  • 6.3.A.1.ii Distinct transfer RNA (tRNA) molecules bind specific amino acids and have anticodon sequences that base pair with the codons of mRNA. tRNA is recruited to the ribosome during translation to generate the primary peptide sequence based on the mRNA sequence.
  • 6.3.A.1.iii Ribosomal RNA (rRNA) molecules are functional building blocks of ribosomes.
• 6.3.A.2 RNA polymerases use a single template strand of DNA to direct the inclusion of bases in the newly formed RNA molecule. This process is known as transcription.
• 6.3.A.3 The enzyme RNA polymerase synthesizes mRNA molecules in the 5' to 3' direction by reading the template DNA strand in the 3' to 5' direction.
• 6.3.A.4 In eukaryotic cells the mRNA transcript undergoes a series of enzyme-mediated modifications.
  • 6.3.A.4.i The addition of a poly-A tail makes mRNA more stable.
  • 6.3.A.4.ii The addition of a GTP cap helps with ribosomal recognition.
  • 6.3.A.4.iii The excision of introns, along with the splicing and retention of exons, generates different versions of the resulting mature mRNA molecule. This process is known as alternative splicing.

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.4.A: Explain how the phenotype of an organism is determined by its genotype.

• 6.4.A.1 Translation of the mRNA to generate a polypeptide occurs on ribosomes that are present in the cytoplasm of both prokaryotic and eukaryotic cells, as well as the cytoplasmic surface of the rough ER of eukaryotic cells.
• 6.4.A.2 In prokaryotic organisms, translation of the mRNA molecule occurs while it is being transcribed.
• 6.4.A.3 Translation involves many sequential steps, including initiation, elongation, and termination. The salient features of translation include:
  • 6.4.A.3.i Translation is initiated when the rRNA in the ribosome interacts with the mRNA at the start codon (AUG, coding for the amino acid methionine).
  • 6.4.A.3.ii The sequence of nucleotides on the mRNA is read in triplets, called codons.
  • 6.4.A.3.iii Each codon encodes a specific amino acid, which can be deduced by using a genetic code chart. Many amino acids are encoded by more than one codon.
  • 6.4.A.3.iv Nearly all living organisms use the same genetic code, which is evidence for the common ancestry of all living organisms.
  • 6.4.A.3.v tRNA brings the correct amino acid to the place specified by the codon on the mRNA.
  • 6.4.A.3.vi The amino acid is transferred to the growing polypeptide chain.
  • 6.4.A.3.vii The process continues along the mRNA until a stop codon is reached.
  • 6.4.A.3.viii Translation terminates with the release of the newly synthesized protein.
  • Exclusion statement: The details and names of the enzymes and factors involved in each of these steps are beyond the scope of the AP Exam.
  • Exclusion statement: Memorization of the genetic code, with the exception of the start codon AUG, is beyond the scope of the AP Exam.
• 6.4.A.4 Genetic information in retroviruses is a special case and has an alternate flow of information: from RNA to DNA, made possible by reverse transcriptase, an enzyme that copies the viral RNA genome into DNA. This DNA integrates into the host genome and is transcribed and translated for the assembly of new viral progeny.

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.5.A: Describe the types of interactions that regulate gene expression.

• 6.5.A.1 Regulatory sequences are stretches of DNA that interact with regulatory proteins to control transcription. Some genes are constitutively expressed, and others are inducible.
• 6.5.A.2 Epigenetic changes can affect gene expression through reversible modifications of DNA or histones.
• 6.5.A.3 The phenotype of a cell or an organism is determined by the combination of genes that are expressed and the levels at which they are expressed.
  • 6.5.A.3.i Observable cell differentiation results from the expression of genes for tissue-specific proteins.
  • 6.5.A.3.ii Induction of transcription factors during development results in sequential gene expression.
  • 6.5.A.3.iii The function and amount of gene products determine the phenotype of organisms.

Learning Objective 6.5.B: Explain how the location of regulatory sequences relates to their function.

• 6.5.B.1 Both prokaryotes and eukaryotes have groups of genes that are coordinately regulated.
  • 6.5.B.1.i Prokaryotes regulate operons in an inducible or repressible system.
  • 6.5.B.1.ii In eukaryotes, groups of genes may be influenced by the same transcription factors to coordinately regulate expression.

Enduring Understanding (BIG IDEA 3 — Information Storage and Transmission): Living systems store, retrieve, transmit, and respond to information essential to life processes.

Learning Objective 6.6.A: Explain how the binding of transcription factors to promoter regions affects gene expression and the phenotype of the organism.

• 6.6.A.1 RNA polymerase and transcription factors bind to promoter or enhancer DNA sequences to initiate transcription. These sequences can be upstream or downstream of the transcription start site.
• 6.6.A.2 Negative regulatory molecules inhibit gene expression by binding to DNA and blocking transcription.

Learning Objective 6.6.B: Explain the connection between the regulation of gene expression and phenotypic differences in cells and organisms.

• 6.6.B.1 Gene regulation results in differential gene expression and influences cell products and functions.
• 6.6.B.2 Certain small RNA molecules have roles in regulating gene expression.