Cell division: count nuclei and chromosomes carefully
| English | 中文 | Pinyin |
|---|---|---|
| chromatid/ˈkrəʊmətɪd/ | 染色单体 | rǎn sè dān tǐ |
| mitotic index/maɪˈtɒtɪk ˈɪndeks/ | 有丝分裂指数 | yǒu sī fēn liè zhǐ shù |
What would explain this observation?
- A root-tip image contains cells at different stages of mitosis. A snapshot can estimate the fraction of cells dividing, but it does not time one observed cell directly.
- Start with a prediction. State the quantities or features you would compare, then decide what evidence could distinguish two explanations.
Build the model
- DNA replication occurs before mitosis. Mitosis separates sister chromatids 染色单体, preserving chromosome number in daughter nuclei. Meiosis includes homologous chromosome separation and normally halves the chromosome number while generating variation.
- mitotic index 有丝分裂指数: Fraction of scored cells undergoing mitosis; chromatid: One copy of a replicated chromosome before separation.
What happens to DNA amount during replication before mitosis?
Count chromosomes by centromeres under the stated convention. DNA amount and chromosome number are different quantities. Crossing over and independent assortment contribute to meiotic variation.
Match each technical term to its precise meaning.
Use the definitions to distinguish related quantities and processes.
Choose evidence that can test it
- Count chromosomes by centromeres under the stated convention. DNA amount and chromosome number are different quantities. Crossing over and independent assortment contribute to meiotic variation.
- Use a prepared root-tip slide or a labelled image. Define a counting rule and field selection before counting. Distinguish dividing cells from damaged or ambiguous cells and report exclusions.
Which two habits make the investigation or model in this case more defensible?
Use a prepared root-tip slide or a labelled image. Define a counting rule and field selection before counting. Distinguish dividing cells from damaged or ambiguous cells and report exclusions.
Work from known quantities
- State the known values and their units. Choose the relation because its assumptions fit this case, then rearrange before substitution.
- Known: 25 dividing cells among 200 scored cells. Mitotic index = 25/200 = 0.125, or 12.5%. Estimating phase duration from this proportion requires assumptions about a steady, representative cell population.
18 of 120 scored cells are dividing. Calculate mitotic index as a percentage. Use the same sequence: known quantities → model → relation → substitution → unit and interpretation.
18 of 120 scored cells are dividing. Calculate mitotic index as a percentage.
The result is 15 %. Known: 25 dividing cells among 200 scored cells. Mitotic index = 25/200 = 0.125, or 12.5%. Estimating phase duration from this proportion requires assumptions about a steady, representative cell population.
Check the conclusion and its limits
- Replication doubles DNA amount without immediately doubling the chromosome count. Meiosis does not simply produce two identical diploid cells.
- Return to the original observation. Explain what the result supports, which conditions it assumes, and one way to test a competing explanation.
DNA replication immediately doubles chromosome number under the centromere convention. This claim is false: Replication doubles DNA amount without immediately doubling the chromosome count. Meiosis does not simply produce two identical diploid cells.
Cell division: count nuclei and chromosomes carefully: Count chromosomes by centromeres under the stated convention. DNA amount and chromosome number are different quantities. Crossing over and independent assortment contribute to meiotic variation.
DNA replication immediately doubles chromosome number under the centromere convention.
Replication doubles DNA amount without immediately doubling the chromosome count. Meiosis does not simply produce two identical diploid cells.
Fraction of scored cells undergoing mitosis: write the technical term.
mitotic index means Fraction of scored cells undergoing mitosis.