필수 실습 6: 실제 식품 염료를 분리 및 식별
도입됨| English | 의미 |
|---|---|
| origin line/ˈɒrɪdʒɪn laɪn/ | 시작 선 |
| solvent front/ˈsɒlvənt frʌnt/ | 용매 전선 |
What would explain this observation?
- An unknown food colouring can contain two known dyes and another dye absent from the reference set. A good result must allow an unmatched spot to remain unmatched.
- Start with a prediction. State the quantities or features you would compare, then decide what evidence could distinguish two explanations.
Build the model
- RP6 investigates separation and comparison of coloured substances and requires Rf calculation. The acquired handbook uses four known food colourings A–D, an unknown U, chromatography paper, water and separate capillary tubes. Its teacher notes suggest U contain two known dyes and a third not in A–D. Known single dyes are preferable references; a commercial colouring may itself be a mixture. Written predictions do not certify that the practical has been performed.
- origin line 起始线: The marked starting line from which chromatographic distances are measured; solvent front 溶剂前沿: The farthest position reached by the moving solvent in the stated chromatogram.
점액된 origin(원점)이 초기 물_level보다 위쪽에 있어야 하는 이유는 무엇인가?
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만약 원점 스팟이 물 سطح 아래에 있었으면 염료들은 종이를 따라 올라가는 대신 저장 탱크로 직접 녹아 들어갔을 것입니다. 용매는 염료를 위로 운반하기 위해 스팟을 관통하여 올라가야 합니다.
각 전문 용어에 대한 정확한 의미를 매칭하시오.
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정의를 사용하여 유사한 양과 과정을 구분하시오.
Choose evidence that can test it
- Draw a horizontal pencil origin 2 cm above the lower paper edge. Mark positions away from the edges, place small concentrated spots using separate clean tubes and label in pencil. The handbook aims for 2–3 mm spots and no more than 1 cm water depth, keeping the origin above the water while the paper bottom dips into it. Support the paper without touching the beaker walls and avoid moving the vessel during the run.
- Stop before the solvent reaches the paper top, remove the paper and mark the solvent front immediately in pencil. Let the paper dry before measuring from the origin to each spot centre and front. Preserve the actual chromatogram, measurements, solvent identity and calculated Rf. Compare U with references in the same run; record an additional spot as unidentified rather than inventing a name. The school method and risk assessment govern capillary handling and any drying equipment.
이 경우 조사 또는 모델이 더 타당하게 만들 수 있는 두 가지 습관은 무엇인가?
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조건과 가정을 명시하면 다른 사람이 정확히 작업을 재현할 수 있으며, 근거 증거를 기록하면 결론을 검증할 수 있습니다. 반대되는 결과를 숨기는 것은 잘못된 결론을 반박할 수 있는 증거를 제거하는 것이며, 한 번의 측정으로는 더 넓은 주장을 입증할 수 없습니다.
Work from known quantities
- State the known values and their units. Choose the relation because its assumptions fit this case, then rearrange before substitution.
- Known: in an original model run the front travels 50 mm. A, B and an unmatched U spot travel 10, 30 and 40 mm respectively. Their Rf values are 0.20, 0.60 and 0.80. If U also has spots at 10 and 30 mm, it supports A and B plus another detectable component. These model readings are practice data; the practical record must contain the student’s real observations.
In an actual run a spot centre travels 33 mm and the front 60 mm from the origin. Calculate Rf. Use the same sequence: known quantities → model → relation → substitution → unit and interpretation.
실제 실험에서 점의 중심이 원점에서 33 mm 이동하고 앞면이 60 mm 이동했습니다. Rf를 계산하십시오.
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결과는 0.55 입니다. 알 수 있는 값: 스팟 중심 33 mm; 원점으로부터 전선 60 mm. Rf = 33/60 = 0.55. 동일한 실험에서 얻은 기준 염료와 비교하면 식별을 뒷받침할 수 있습니다.
Check the conclusion and its limits
- Ink can move and interfere, so the baseline and labels use pencil. An origin submerged in solvent lets samples dissolve into the reservoir. Large spots can overlap; touching walls can distort the front. No spot matching A–D does not mean the unknown contains no dye. RP6 applies on both tiers and assesses actual separation skills alongside written interpretation.
- Return to the original observation. Explain what the result supports, which conditions it assumes, and one way to test a competing explanation.
A written chromatography calculation alone proves RP6 was completed. This claim is false: The practical assesses real observations - an actual chromatogram with measured distances. A written Rf calculation from invented numbers demonstrates arithmetic, not that the practical was performed.
Required practical 6: separate and identify actual food dyes: Draw a horizontal pencil origin 2 cm above the lower paper edge. Mark positions away from the edges, place small concentrated spots using separate clean tubes and label in pencil. The handbook aims for 2–3 mm spots and no more than 1 cm water depth, keeping the origin above the water while the paper bottom dips into it. Support the paper without touching the beaker walls and avoid moving the vessel during the run.
크로마토그래피 계산서 하나만으로 RP6이 완료되었음을 증명할 수 있다.
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실습 평가는 실제 관측 결과를 반영합니다 - 거리가 측정된 실제 크로마토그램입니다. 발명된 숫자를 이용한 서면 Rf 계산은 산술 능력을 보여줄 뿐, 실습을 수행했음을 증명하지는 않습니다.
크로마토그래피 거리를 측정하는 기준이 되는 표시된 시작 선: 기술 용어를 쓰시오.
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origin line(origin 라 인)은 크로마토그래피 거리를 측정하는 기준이 되는 표시된 시작 선을 의미한다.