Pearson Edexcel · International A-Level · Biology

A red blood cell leaving your lung carries oxygen on haemoglobin, rides an artery, squeezes through a capillary narrower than itself, releases the oxygen to a respiring cell, and returns through a vein. Every structure in that journey is built from the molecules this unit starts with, and every exam question about it asks you to connect structure to function.

WBI11 Molecules, Diet, Transport and Health is the first IAS unit: a written paper of 1 hour 30 minutes, 80 marks, all compulsory. It assesses Topic 1 (Molecules, Transport and Health) and Topic 2 (Membranes, Proteins, DNA and Gene Expression). Expect data questions on health studies, calculations with standard form, and extended answers that name structures and explain mechanisms.

Water is the transport medium because it is a polar solvent 极性溶剂. In a water molecule, oxygen pulls the shared electrons more strongly than hydrogen: each O–H bond has a slight negative end at O and a slight positive end at H. This is the dipole 偶极 nature of water. Opposite charges between neighbouring molecules form hydrogen bonds 氢键, and polar or charged particles (glucose, sodium ions, amino acids) dissolve because water clusters around them.

Carbohydrates are joined and split by two reactions:

• Kondensasi 缩合 removes a water molecule as a glycosidic bond 糖苷键 forms between two sugars.
• Hidrolisis 水解 adds water back and breaks that bond.

Two glucose molecules condense to maltose; glucose plus fructose give sucrose; glucose plus galactose give lactose. Long chains make polysaccharides: amylose 直链淀粉 (1,4-linked, coils into a helix, compact), amylopectin 支链淀粉 dan glycogen 糖原 (both 1,4-chains with 1,6-branches; glycogen is the animal store in liver and muscle). Branching means many chain ends, so glucose can be released quickly; the helix means a lot of energy fits in a small space.

Core Practical 1: estimating sugar and starch concentration

Reducing sugars reduce blue Benedict's reagent on heating, giving a brick-red precipitate. To estimate an unknown concentration, compare the colour against standards of known concentration, or read the solution in a colourimeter.

Worked check. Standards of 0, 0.5, 1, 2 and 4 % glucose give absorbances 0.02, 0.09, 0.18, 0.35, 0.64. An unknown gives 0.58. On the straight section, each 1 % adds about 0.16 absorbance, so the unknown is near $0.58/0.16 \approx 3.6\%$. Reading beyond the standards (extrapolating past 4 %) is less reliable than measuring within them — a point the examiners like you to make. Iodine turns blue-black with starch; intensity of the blue-black gives the same kind of estimate for starch.

A triglyceride 甘油三酯 is one glycerol plus three fatty acids, joined by three condensation reactions that form ester bonds 酯键. Saturated 饱和 fatty acids have no C=C bonds: straight chains pack closely, so animal fats are solid at room temperature. Unsaturated 不饱和 acids have one or more double bonds that kink the chain, packing is looser, and oils are liquid. Lipids store more energy per gram than carbohydrates because they are more reduced.

Diffusion supplies cells only a few tenths of a millimetre thick. A big organism needs mass transport 大规模运输: a pump and pipes that carry every cell's supply and waste. The three vessel types match three jobs:

The mammalian heart is two pumps in one organ. The right side sends blood to the lungs; the left side, with the thicker ventricle wall, sends blood to the body. Atria receive; ventricles deliver. The coronary arteries feed the heart muscle itself — blockage there is a heart attack.

Siklus jantung

One beat has three phases. Sistol serambi 心房收缩期 squeezes the last blood into the ventricles. Sistol ventrikel 心室收缩期 raises ventricular pressure above arterial pressure, the semilunar valves open, and blood is driven out. Diastole 舒张期 relaxes the muscle, pressure falls, the semilunar valves slam shut (the second heart sound), and the chambers refill from the veins.

Read the figure the way an examiner wants: valve state is decided by pressure difference, never by an instruction. Atrio-ventricular valves open when atrial pressure exceeds ventricular pressure; semilunar valves open when ventricular pressure exceeds aortic pressure.

Haemoglobin is a protein of four polypeptide chains, each folded around a haem group carrying one Fe²⁺ ion that binds one O₂ molecule — four in total. Binding the first oxygen changes the shape of the whole molecule and makes the next bindings easier: cooperative binding 协同结合 gives the oxygen dissociation curve its shallow-then-steep S shape.

In the lungs (high pO₂) the curve flattens near 100 % saturation. In resting tissue near 1–2 kPa the curve is steep, so a small fall in pO₂ unloads a large amount of oxygen — exactly where it is needed. When tissue respires hard it releases more carbon dioxide; hydrogen ions attach to haemoglobin and shift the whole curve to the right (the Bohr effect 波尔效应), forcing even more oxygen to unload in the busiest tissue.

Worked check. At pO₂ 2 kPa the normal curve reads about 30 % saturation and the shifted curve about 15 % — haemoglobin holds only about a third as much oxygen at that partial pressure, so most of its four sites are released to the tissue sooner.

Atherosclerosis 动脉粥样硬化 begins with damage to the endothelium 内皮 lining an artery. The inflammatory response grows, low-density lipoproteins invade, smooth muscle and connective tissue build a plaque 斑块, the artery narrows and hardens, and a clot can finally block it or break loose.

Clotting itself is a cascade. Damage releases thromboplastin 凝血酶原激酶, which (with calcium ions) converts the soluble plasma protein prothrombin 凝血酶原 into the enzyme thrombin 凝血酶; thrombin converts soluble fibrinogen 纤维蛋白原 into the insoluble mesh of fibrin 纤维蛋白 that traps platelets and cells.

Blood pressure 血压 is reported as systolic over diastolic. Hypertension, smoking, high blood LDL-cholesterol, diet, inactivity, age, gender and genetics are risk factors 危险因素 — and they multiply, not add: two moderate risks can matter more than one severe risk.

Reading health-risk data

Questions 8 and 9 of every paper live here. Four habits cover almost every mark:

• Correlation is not causation. 相关不等于因果 Two variables moving together may share a hidden cause, or the link may be chance.
• Judge the study: How large was the sample? How was it selected? What was controlled? Was it blind? Are there confounders 混杂变量 such as age or smoking?
• Do the arithmetic carefully: percentage change, ratios in standard form, per-capita rates.
• Ask who or what is missing: non-responders, unpublished studies, extrapolated lines.

Worked check. A study finds gum disease correlates with endothelial damage. Before claiming one causes the other you would want a mechanism, a dose–response pattern, and confounders (diet, smoking, age) excluded — and the question "were the groups matched for blood pressure?" is exactly a confounder question.

Energy balance decides body mass. Overweight and obesity are screened by BMI (mass in kg divided by height in metres squared; 18.5–24.9 is the healthy band) and waist-to-hip ratio (WHR). Dietary saturated fat raises blood LDL-cholesterol; unsaturated fat and soluble fibre lower it.

Worked check. A woman of mass 74 kg and height 1.66 m: BMI $= 74 / 1.66^2 = 74/2.76 = 26.8$ — just into the overweight band. Waist 84 cm and hips 98 cm give WHR $= 84/98 = 0.86$.

Treatments all carry risks alongside benefits:

• Antihypertensives 抗高血压药 (beta blockers, ACE inhibitors, diuretics) lower blood pressure.
• Statins 他汀类 (for example simvastatin) block a liver enzyme in cholesterol synthesis, so cells take up LDL from blood.
• Anticoagulants 抗凝剂 (warfarin) and antiplatelet drugs (aspirin) reduce clotting, so bleeding risk rises.

A question asking you to "discuss" wants both sides plus a judgement anchored in the data given.

Every exchange surface is a membrane doing controlled business. The fluid mosaic model 流动镶嵌模型 is the accepted picture: a phospholipid bilayer (fluid because the tails can slide), studded with intrinsic proteins that span the bilayer (channels, carriers), extrinsic proteins, cholesterol that controls fluidity, and glycoproteins and glycolipids for recognition.

Four transport routes:

• Difusi 扩散 — net movement down a concentration gradient, no ATP.
• Facilitated diffusion 易化扩散 — down the gradient through a channel or carrier protein.
• Transportasi aktif 主动运输 — against the gradient, powered by ATP, through carrier proteins that change shape.
• Osmosis 渗透 — water moving from higher to lower water potential 水势 across a partially permeable membrane. Endocytosis and exocytosis move bulk material in vesicles.

For diffusion, Fick's law: rate is proportional to (surface area × concentration difference) ÷ diffusion distance. Gas exchange surfaces answer all three: huge area, maintained gradient, and a wall one or two cells thick. This is why alveoli, capillaries and fish gills all look the way they do.

An amino acid carries an amino group, a carboxyl group, a hydrogen and an R group on one central carbon; two join by a peptide bond 肽键 in a condensation reaction. The primary structure 一级结构 is the sequence; hydrogen bonds coil it into the sekunder 二级结构; further hydrogen, ionic and disulfide bonds fold the tertiary 三级结构; separate chains assemble into the quaternary 四级结构 — haemoglobin is the classic example.

An enzyme 酶 is a protein whose active site 活性部位 fits one substrate shape. Binding is not rigid: the induced-fit 诱导契合 model has both molecule and site flexing until complementary. This is why temperature and pH change rate — they act on the tertiary structure — and why the active site is specific.

Competitive inhibitors 竞争性抑制剂 bind the active site and are outnumbered as substrate concentration rises; non-competitive inhibitors 非竞争性抑制剂 bind elsewhere and distort the site, so rate falls whatever the concentration.

Worked check. Q10 is quoted at 2.4: between 10 °C and 20 °C the rate is 2.4 times faster; between 45 °C and 55 °C the same factor means the denatured enzyme is collapsing at an increasing rate. Q10 only describes; it does not explain.

A mononucleotide 单核苷酸 is a pentose (deoxyribose or ribose), a phosphate and a nitrogenous base. Condensation between phosphate and sugar of neighbours makes a strand held by phosphodiester bonds 磷酸二酯键. DNA is two antiparallel strands: A pairs with T (two hydrogen bonds), G with C (three), twisted into a double helix.

Replication is semi-conservative 半保留: helicase unzips the two strands, each is a template, and DNA polymerase DNA聚合酶 joins free nucleotides in the 5' to 3' direction. Meselson and Stahl grew bacteria first on heavy ¹⁵N then light ¹⁴N nitrogen; after one round of replication all the DNA was one intermediate density, after two rounds half was light — exactly what semi-conservative predicts and the alternatives forbid.

A gene is a sequence of bases coding for a sequence of amino acids. The code is a triplet 三联体 code: three bases per amino acid. It is non-overlapping 非重叠 (read in successive groups), degenerate 简并 (most amino acids have several codons, so some mutations are silent), and carries start and stop codons.

Worked check. A gene of 300 base pairs coding a protein of 99 amino acids plus a stop codon: $99 \times 3 + 3 = 300$. ✓

Transcription: RNA polymerase binds the gene, uses one strand as template and builds mRNA 信使RNA. Translation: in the cytoplasm, a ribosome 核糖体 reads the mRNA codons, and tRNA 转运RNA molecules whose anticodons 反密码子 pair with those codons deliver amino acids in order; peptide bonds join them.

Mutasi 突变 arise from replication errors. A substitution 替换 swaps one base: one amino acid changes, or nothing changes (degeneracy). An insertion 插入 atau deletion 缺失 adds or removes a base and shifts every reading frame after it — a frameshift 移码 — usually destroying the protein.

Cystic fibrosis is the course's model: deletion of three bases removes one phenylalanine from the CFTR protein, so chloride transport fails, mucus stays thick, and airways clog. The allele is recessive: carriers are healthy. Genetic screening 基因筛查 can identify carriers, test embryos (pre-implantation genetic diagnosis) or foetuses (amniocentesis, chorionic villus sampling). Screening questions always carry ethics: who is tested, who is told, what happens to a positive result.

1. Explain why amylopectin releases glucose faster than amylose, referring to bonds and ends of chains.
2. Calculate the BMI of a 91 kg man 1.83 m tall and classify him.
3. During ventricular systole, which valves are open and which are shut, and why?
4. The oxygen saturation at 4 kPa falls from about 60 % to about 34 % when CO₂ rises. Explain the molecular cause.
5. Describe the clotting cascade in order, from endothelial damage to fibrin.
6. A study reports that people who eat more yoghurt have fewer heart attacks. List three checks you would make before believing a causal claim.
7. Explain why a competitive inhibitor lowers the rate at low substrate concentration but not at very high concentration.
8. Meselson and Stahl ruled out conservative replication. Which observation, after one round, did the job?
9. A frameshift mutation is usually worse than a substitution. Explain why, using the words triplet and degenerate.
10. Name two benefits and two risks of genetic screening for cystic fibrosis carriers.

Answers: 1 many 1,6-branch points give many chain ends for enzymes to attack simultaneously; 2 $91/1.83^2 = 27.2$, overweight; 3 atrio-ventricular shut, semilunar open, because ventricular pressure exceeds aortic but not atrial-from-above pressure; 4 hydrogen ions from CO₂ bind haemoglobin, changing its shape and lowering oxygen affinity (Bohr effect); 5 damage exposes collagen, platelets and damaged cells release thromboplastin, thromboplastin + Ca²⁺ converts prothrombin to thrombin, thrombin converts fibrinogen to fibrin, fibrin mesh traps cells; 6 sample size and selection, confounders such as age/exercise/diet, blinded comparison, mechanism or dose–response; 7 the inhibitor competes for the active site, so at very high substrate concentration nearly every collision with the site is substrate; 8 after one round all DNA was a single intermediate band — conservative predicts one heavy and one light band; 9 a frameshift changes every triplet after the mutation, so the whole protein from that point is a different amino-acid sequence, while degeneracy lets many substitutions change nothing; 10 benefits — informed family planning, earlier treatment or lifestyle change; risks — anxiety, discrimination by insurers or employers, false positives or negatives.

You began as one fertilised egg cell. This unit explains how that cell divided, how the copies were shuffled and halved to make gametes, how identical genes became different tissues, and how the variety that produces is counted, classified and — increasingly — defended. It is the unit of microscopes, calculations and definitions done precisely.

WBI12 Cells, Development, Biodiversity and Conservation is the second IAS unit: 1 hour 30 minutes, 80 marks, all compulsory. It assesses Topic 3 (Cell Structure, Reproduction and Development) and Topic 4 (Plant Structure and Function, Biodiversity and Conservation). Expect labelling diagrams, microscope and magnification calculations, the index of diversity and Hardy–Weinberg questions, and long answers on conservation evaluation.

A eukaryotic 真核 cell is a set of specialised compartments:

Cells organise into tissues 组织 (similar cells), organs 器官 (cooperating tissues) and organ systems 器官系统. A prokaryotic 原核 cell has none of the membrane-bound organelles: a cell wall, a slime capsule 荚膜, one circular DNA molecule plus plasmids 质粒, 70S ribosomes, and sometimes a flagellum 鞭毛.

Pembesaran 放大倍数 is how much bigger the image is; resolution 分辨率 is the smallest separation still seen as two. Light microscopes resolve about 200 nm; electron beams, far shorter in wavelength, resolve to a few nanometres — but need thin stained sections (TEM) or gold-coated surfaces (SEM). $M = \text{image size} / \text{actual size}$, keeping units consistent.

Worked check. A mitochondrion measures 14 mm on a photograph labelled ×20 000. Actual length $= 14\,000\ \mu\text{m} / 20\,000 = 0.7\ \mu$m. A nucleus that must be seen with its double membrane needs an electron microscope: the two membranes lie closer than a light microscope's resolution.

Meiosis 减数分裂 is two successive divisions after one DNA replication, producing four genetically different haploid 单倍体 cells from one diploid 二倍体 cell. Two processes create the variation:

• Penataan bebas 独立分配 — each homologous pair lines up and separates at random in meiosis I; each gamete gets one of each pair, from either parent at random ($2^n$ combinations).
• Percilangan silang 交叉互换 — in prophase I, homologous chromatids swap sections, mixing alleles within chromosome pairs.

Mammalian gametes are specialised for delivery and provisioning: the sperm 精子 carries an acrosome 顶体 (digestive enzymes), a midpiece of mitochondria and a flagellum; the egg 卵子 contributes nearly all cytoplasm and a zona pellucida 透明带 that locks out extra sperm after the cortical reaction 皮质反应. In flowering plants the pollen tube delivers two male nuclei: one fertilises the egg, the other the polar nuclei — double fertilisation 双受精.

Mitosis 有丝分裂 produces two genetically identical diploid cells: growth, repair, asexual reproduction. Interphase ($G_1$, S — DNA replication, $G_2$) fills most of the cycle; mitosis itself is prophase (chromosomes condense, spindle forms), metaphase (chromosomes line at the equator), anaphase (spindle fibres pull chromatids to poles), telophase (nuclear membranes reform), then cytokinesis 胞质分裂.

The mitotic index 有丝分裂指数 $=$ cells in mitosis $\div$ total cells counted. High values in a tissue biopsy mean rapid division — a cancer signature.

Worked check. A root-tip squash shows 36 of 450 cells in mitosis: index $= 36/450 = 0.08 = 8\%$. If the cycle takes 20 hours, mitosis occupies $0.08 \times 20 = 1.6$ hours.

A totipotent 全能性 stem cell can form a whole organism (the first divisions of the zygote); a pluripotent 多能性 cell (inner blastocyst 胚泡) can form any tissue but not the whole organism. Differentiation happens by differential gene expression 差异基因表达: every cell keeps the whole genome, but each type switches on only its own subset. One gene can yield several proteins by post-transcriptional modification 转录后修饰 — RNA splicing choices of the pre-mRNA. The final phenotype also reflects the environment (height needs both genes and nutrition) and, for many traits, multiple genes acting together (polygenic 多基因 inheritance giving continuous variation).

Plant cells add a cellulose cell wall 细胞壁 joined to neighbours by plasmodesmata 胞间连丝. Cellulose chains bundle into microfibrils 微纤维 whose criss-cross laying gives tensile strength; secondary thickening with lignin strengthens and waterproofs.

Water and ions matter beyond drinking: nitrate 硝酸盐 builds amino acids, kalsium 钙离子 cross-links pectin in walls and signals inside cells, magnesium 镁离子 sits at the heart of chlorophyll — a shortage yellows the leaves.

Classification arranges life by shared characteristics; the three-domain system 三域系统 (Bacteria, Archaea, Eukarya) rests on molecular evidence — ribosomal RNA sequences — that revealed Archaea are closer to us than to bacteria. Within a habitat, diversity has two measures:

• Species richness 物种丰富度 — how many species.
• Index of diversity 多样性指数 — $D = \dfrac{N(N-1)}{\sum n(n-1)}$, where $N$ is all individuals and $n$ each species' count. Higher $D$, more diverse.

Worked check. Habitat A: 3 species with counts 40, 35, 25 ($N = 100$). $D = 100 \times 99 / (40 \times 39 + 35 \times 34 + 25 \times 24) = 9900 / (1560 + 1190 + 600) = 9900/3350 = 2.96$. Habitat B: 3 species, counts 90, 6, 4: $D = 9900/(8010 + 30 + 12) = 1.23$. Same richness, very different balance — $D$ sees what richness cannot.

Within one species, the heterozygosity index 杂合度指数 $H =$ (number of heterozygotes) $\div$ (number of individuals) measures genetic variety. A species found nowhere else is endemic 特有种 — and its island home makes it both precious and fragile.

A niche 生态位 is a species' way of life: its role, its requirements, its tolerances. Adaptations come in three registers — anatomical (thick fur), physiological (enzyme variants), behavioural (migration timing).

The Hardy–Weinberg equation 哈迪–温伯格方程 tracks allele frequencies in a large, randomly breeding population with no selection, migration or mutation:

$q^2$ = frequency of the homozygous recessive genotype. If 4 % of a population shows the recessive phenotype, $q^2 = 0.04$, so $q = 0.2$, $p = 0.8$, carriers $2pq = 0.32$.

Worked check. Of 500 foxes, 45 are homozygous recessive: $q^2 = 0.09$, $q = 0.3$, $p = 0.7$. Expected carriers $= 2pq \times 500 = 0.42 \times 500 = 210$.

Conservation questions want balance. Zoos and seed banks 种子库 keep species against extinction and run captive breeding, but cost space, lose natural behaviour and serve few individuals; habitat protection and corridors 走廊 preserve ecology at scale but need land, money and political will. Credit the specific argument, not the sentiment.

For the microbiology here (and Unit 4's): grow bacteria on nutrient agar or in broth, using aseptic technique 无菌技术 — sterilise loops by flaming, flame bottle necks, work near an updraught, seal plates, incubate below body temperature so human pathogens cannot grow. Count colonies on spread or pour plates (each colony from one cell or a clump: colony-forming units), or count cells with a haemocytometer, or follow turbidity 浊度 in broth.

Worked check. In the exponential phase, the growth-rate constant $k = (\log_{10} N_t - \log_{10} N_0)/(0.301 \times t)$. From $1 \times 10^4$ ke $4 \times 10^7$ cells in 4 hours: $\log$ difference $= 3.602$, so $k = 3.602/(0.301 \times 4) \approx 3.0$ divisions per hour — about a 20-minute generation time.

1. Calculate the actual width of a nucleus that appears 24 mm wide at ×4 000. Give your answer in µm.
2. Name the two processes in meiosis that create genetic variation, and state when each happens.
3. A cell has 2 a.u. of DNA in G1. Sketch how DNA content changes through S phase, meiosis I and meiosis II.
4. Explain why all cells of a blastocyst can become any tissue, but a mature muscle cell cannot.
5. Explain two ways xylem is adapted to its function, one structural and one chemical.
6. Two habitats each contain 5 plant species. Explain why the index of diversity can still differ between them.
7. In a population of 1 000, 16 people show a recessive condition. Calculate q, p and the expected number of carriers.
8. A species of snail is endemic to one island. Explain why endemic species face higher extinction risk.
9. State three conditions a population must meet for the Hardy–Weinberg equation to hold.
10. Give one benefit and one limitation of seed banks compared with protecting habitat.

Answers: 1 $24\,000/4\,000 = 6\ \mu$m; 2 crossing over in prophase I, independent assortment in metaphase/anaphase I (and II); 3 rises to 4 in S, halves to 2 after meiosis I, halves to 1 after meiosis II, restored to 2 at fertilisation; 4 blastocyst cells are pluripotent — all genes still switchable; a muscle cell has switched off other pathways permanently in ordinary conditions; 5 hollow, lignified dead cells form continuous water columns with no cytoplasm to resist flow; lignin waterproofs and strengthens walls against collapse; 6 D weighs the evenness of abundance, not just the species count; 7 $q^2 = 0.016$, $q \approx 0.126$, $p \approx 0.874$, carriers $2pq \approx 0.22$, about 220 people; 8 a single local event (storm, disease, introduced predator, habitat loss) removes the whole species at once; 9 large population, random mating, no selection/mutation/migration; 10 seed banks store genetic diversity cheaply and safely against habitat loss (benefit) but species exist out of their ecology — no interactions, no evolution in place, and recolonisation is uncertain (limitation).

A wheat field intercepts sunlight, fixes carbon, feeds a food chain — and loses energy at every step. This unit follows the energy from photon to ecosystem, then turns to the smallest participants: the microbes that recycle, the pathogens that take, and the immune system that defends. WBI14 is also the most calculation-heavy paper after Unit 1: expect NPP, growth constants, diversity indices and time-of-death arithmetic.

WBI14 Energy, Environment, Microbiology and Immunity is the first IA2 unit: 1 hour 30 minutes, 90 marks, all compulsory. It assesses Topic 5 (Energy Flow, Ecosystems and the Environment) and Topic 6 (Microbiology, Immunity and Forensics).

The overall reaction: $6\mathrm{CO_2} + 6\mathrm{H_2O} \rightarrow \mathrm{C_6H_{12}O_6} + 6\mathrm{O_2}$, driven by light energy. In the thylakoid 类囊体 membranes, the light-dependent reactions 光反应 photolyse water (releasing O₂ and H⁺), photophosphorylate 光合磷酸化 ADP and reduce NADP. In the stroma 基质, the light-independent reactions 暗反应 (Calvin cycle) fix carbon dioxide onto ribulose bisphosphate with rubisco, reduce the products using the ATP and reduced NADP from stage one, and make GALP — some to glucose, most to regenerate RuBP.

Structure matches function: thylakoids stacked for absorbing surface; stroma packed with enzymes for the cycle.

Pigments and spectra

Chlorophyll a and b absorb red and blue light; carotenoids widen the range and protect. The absorption spectrum 吸收光谱 shows what each pigment takes up; the action spectrum 作用光谱 shows what rate of photosynthesis each wavelength drives — their agreement is evidence that these pigments do the work. Chromatography separates pigments by solubility; $\mathrm{Rf} = \text{distance moved by pigment} / \text{distance moved by solvent}$.

Faktor pembatas 限制因素: light intensity, CO₂ concentration, temperature. A greenhouse raises the weakest of the three — and exam answers say which and why.

Gross primary productivity 总初级生产力 (GPP) is the energy fixed by photosynthesis per area per time (kJ m⁻² yr⁻¹). Plants spend much of it on their own respiration; what remains is net primary productivity 净初级生产力:

Only about 10 % of the energy at each trophic level passes upward — lost to respiration as heat, egestion, excretion and parts never eaten. That is why chains rarely exceed four or five levels and why top predators are rare.

Worked check. A grassland fixes 45 000 kJ m⁻² yr⁻¹ and respirers 19 000. NPP $= 26\,000$ kJ m⁻² yr⁻¹. Cattle eating that grass keep about 10 %: 2 600 kJ m⁻² yr⁻¹. Humans eating the cattle keep 260 — the energy arithmetic behind "eating lower on the chain feeds more people".

Four definitions, precisely: habitat 栖息地 (where), population 种群 (one species' individuals), community 群落 (all populations), ecosystem 生态系统 (community + environment). Abiotic factors (light, water, temperature, pH) and biotic factors (competition, predation, disease) set who lives where and how abundantly — together, the niche 生态位.

Predator and prey cycle with a delay: hares rise, lynx follow, hares fall, lynx starve — a lag of about a quarter cycle.

Succession 演替 begins when pioneer species colonise bare ground; each stage changes the soil and microclimate, enabling the next, until the climax community 顶极群落 stabilises. Measuring abundance uses quadrats and transects (random sampling for estimates, systematic along a gradient) and capture-mark-recapture for animals ($N \approx \text{first catch} \times \text{second catch} / \text{marked recaptures}$).

Evidence comes from direct temperature records, ice cores (trapped air giving ancient CO₂), tree rings (dendrochronology 树轮年代学) and pollen layers in peat. The enhanced greenhouse effect 温室效应: outgoing long-wave radiation is absorbed by CO₂, methane and nitrous oxide and re-emitted downward. The gases differ — methane is potent but short-lived; CO₂ lasts centuries; nitrous oxide combines both — so policy weighs global warming potential 全球增温潜势 against lifetime.

Extrapolating a trend beyond its data is a judgement, not a fact: state the assumption (the trend continues) and its weakness. Effects already examined: shifting rainfall and species ranges, and — close to this course — temperature's effect on enzyme rates in cold-blooded organisms and whole ecosystems.

Worked check. Methane's warming potential is about 28× CO₂'s per kg, but it lasts ~12 years against centuries. A one-tonne methane leak matters 28× a one-tonne CO₂ leak this decade, but fades while the CO₂ persists — the arithmetic of "warming potential vs cumulative load".

Evolution is a change in allele frequency 等位基因频率. It needs variation (mutation, meiosis) and a filter: directional selection 定向选择 shifts the mean when the environment moves; stabilising selection 稳定选择 trims the extremes in a stable one. Drift changes frequencies by chance in small populations; gene flow (migration) mixes them back.

Spesiasi 物种形成 needs isolation: allopatric 异域的 (a physical barrier — allopatric = different homeland) or sympatric 同域的 (reproductive isolation in the same place — behavioural, temporal or genetic). Isolated populations diverge until they can no longer interbreed.

Aseptic technique protects you and the culture: flame the loop to red heat, flame bottle necks, lift lids briefly, seal plates with tape, incubate at 25 °C (never body temperature, so human pathogens cannot multiply). Measure growth by direct cell count (haemocytometer), turbidity 浊度 in broth, or viable counts by dilution plating 稀释涂布 (colonies counted × dilution factor).

The growth curve: lag 延迟期 (enzymes synthesising), log 对数期 (exponential doubling), stationary 稳定期 (deaths = divisions), death 衰亡期. The exponential constant:

Worked check. From $2\times10^4$ ke $1.6\times10^7$ cells in 5 hours: log difference $= 2.903$; $k = 2.903/(0.301\times5) = 1.93$ generations per hour — a doubling time of about 31 minutes.

Bacteria: cell wall, plasmids, ribosomes, circular DNA, some with capsule and flagellum. Viruses: nucleic acid (DNA or RNA) in a capsid 衣壳, some with an envelope — no organelles, no metabolism of their own; they hijack host cells. Mycobacterium tuberculosis 结核分枝杆菌 infects lungs, surviving inside macrophages; HIV 人类免疫缺陷病毒 destroys T helper cells, so the immune system collapses and TB reactivates — the classic AIDS-defining illness.

Barriers come first: skin, mucus and cilia, stomach acid, lysozyme in tears, and the clotting cascade sealing wounds.

Non-specific: inflammation (vasodilation and fluid leak bringing phagocytes), phagocytosis, interferon. Specific — antigens 抗原 trigger it:

• Humoral 体液: B effector cells → plasma cells → antibodies 抗体; B memory cells B记忆细胞 remain for the faster, stronger secondary response.
• Cellular 细胞: T helper cells coordinate, T killer cells T杀伤细胞 destroy infected cells, T memory cells persist.

An antibody is four polypeptide chains (two heavy, two light) with a variable region 可变区 forming one specific binding site — the shape that fits one antigen. Immunity splits two ways: aktif 主动 (your own antibodies — natural infection or vaccination) vs passive 被动 (given antibodies — mother to baby, antiserum); natural vs artificial in each. Vaccination works at the scale of populations too (herd immunity 群体免疫) — but pathogens evolve back: the evolutionary race.

Worked check. A vaccine's second dose produces antibody levels ten times the first and within days rather than weeks: clonal selection finds the memory B cells, which divide rapidly into plasma cells — the secondary response the primary response built.

Bacteriostatic 抑菌的 antibiotics stop bacterial growth; bactericidal 杀菌的 ones kill. Resistance arises by mutation and spreads by selection whenever exposure kills the susceptible and spares the resistant — finishing a course and avoiding unnecessary use both slow it. Hospital-acquired infections thrive where vulnerable patients, invasive devices and resistant strains meet; hand hygiene, sterile procedure and isolating carriers break the chain.

Decomposers recycle carbon and nitrogen — the ecosystem's waste system. The polymerase chain reaction 聚合酶链式反应 cycles denaturation (95 °C), primer annealing (50–60 °C) and extension (72 °C), doubling the target DNA each cycle: $n$ cycles give $2^n$ copies. Gel electrophoresis 凝胶电泳 drags negatively charged DNA fragments through gel — short fragments run furthest. The profile 电泳图谱 of band positions identifies individuals (enough loci differ between unrelated people) and measures relatedness (shared bands).

Time of death narrows by four clocks: body temperature (falls ~1 °C per hour, modified by size, clothing, air), rigor mortis 尸僵 (sets then passes), decomposition degree, and insect succession on the body — each stage's arrivals time-stamp the interval.

Worked check. A body found at 22 °C core temperature in a 15 °C room: $(37-22)/(1\ ^\circ\text{C per hour}) \approx 15$ hours — but state the assumptions (still air, average build) before trusting it.

1. Name the products of the light-dependent reactions used by the Calvin cycle.
2. A crop fixes 60 000 kJ m⁻² yr⁻¹ and respirers 20 000. Calculate NPP and the energy reaching a third trophic level at 10 % transfer each step.
3. Explain why the action spectrum is evidence that chlorophyll carries out photosynthesis.
4. Distinguish directional and stabilising selection with one example of each.
5. Give two reasons a food chain rarely exceeds five trophic levels.
6. A culture grows from $5\times10^3$ ke $4\times10^6$ in 6 hours. Calculate k and the doubling time.
7. Explain why a second vaccine dose raises antibody titre far faster than the first.
8. State the difference between bacteriostatic and bactericidal, and why the distinction matters for a patient with a weak immune system.
9. Three PCR cycles from one double-stranded template: how many copies?
10. A body's core is 30 °C. Estimate the time since death under standard assumptions and name one factor that would extend the true interval.

Answers: 1 ATP and reduced NADP; 2 NPP $= 40\,000$; third level $= 400\,\text{kJ m}^{-2}\text{yr}^{-1}$ (10 % of 4 000); 3 wavelengths chlorophyll absorbs best are the wavelengths that drive photosynthesis best; 4 directional — antibiotic resistance shifting the mean; stabilising — human birth weights; 5 energy lost at each step to respiration/heat/egestion, so little remains; producers also lose to respiration before the first transfer; 6 log difference 2.903, $k = 2.903/(0.301\times6) = 1.61$ h⁻¹, doubling ≈ 37 min; 7 memory B cells persist; the second encounter selects clones that divide rapidly into plasma cells — the secondary response; 8 bacteriostatic stops growth (immune system must clear), bactericidal kills outright — a weak immune system needs bactericidal support; 9 $2^3 = 8$ copies; 10 $(37-30)/1 = 7$ hours; heavy clothing or a warm room slows cooling, so true time is longer.