Learn Extracted exam questions A-Level Biology 9700 Biology November 2025 Question Paper 35
9700 Biology November 2025 Question Paper 35
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1 Dialysis tubing is a partially permeable membrane. Some molecules such as glucose molecules can diffuse through pores in the membrane.
You are required to investigate the diffusion of glucose through the pores in dialysis tubing using two different concentrations of glucose.
You are provided with the materials shown in Table 1.1. Table 1.1 labelled contents hazard volume / cm3 R 20.0% glucose solution low 20 S 10.0% glucose solution low 20 G 1.0% glucose solution low 30 W distilled water low 100 Benedict’s Benedict’s solution harmful irritant 20 D1 length of dialysis tubing in distilled water low – D2 length of dialysis tubing in distilled water low –
If any solution comes into contact with your skin, wash off immediately with cold water. It is recommended that you wear suitable eye protection.
You will need to: • put two different concentrations of glucose solution into dialysis tubing surrounded by water • take a sample of the water surrounding the dialysis tubing • test the sample for the presence of glucose. , ,
Carry out step 1 to step 10.
step 1 Draw a mark 8 cm from the top of a large test‑tube, as shown in Fig. 1.1. 8 cm mark 8 cm from the top Fig. 1.1
step 2 Remove the dialysis tubing from beaker D1. Tie a knot in the dialysis tubing as close as possible to one end, so that the end is sealed.
step 3 The whole length of the dialysis tubing needs to be separated to allow the tubing to be filled with solution. To do this, rub the whole length of the dialysis tubing gently between your finger and thumb.
step 4 Put 10 cm3 of 20.0% glucose solution, R, into the open end of the dialysis tubing.
step 5 Rinse the outside of the dialysis tubing by dipping it in the water in beaker D1.
step 6 Put the dialysis tubing containing R into the large test‑tube and keep it in position using an elastic band as shown in Fig. 1.2. mark on test-tube 10 cm3 of glucose solution knot elastic band large test-tube dialysis tubing Fig. 1.2 , ,
step 7 Put distilled water into the large test‑tube so that the top of the water is above the level of the glucose solution in the dialysis tubing.
step 8 Start timing and leave the dialysis tubing in the distilled water for 15 minutes.
step 9 Repeat step 1 to step 7 using the dialysis tubing in the container labelled D2 and the 10.0% glucose solution, S, instead of R.
step 10 Start timing and leave the dialysis tubing in the distilled water for 15 minutes.
While you are waiting, continue with preparing the glucose standards.
Preparing glucose standards
You will need to carry out a serial dilution of the 1.0% glucose solution, G, to reduce the concentration by half between each successive dilution.
You will need to prepare four concentrations of glucose solution in addition to the 1.0% glucose solution, G.
After the serial dilution is completed, you will need to have 10 cm3 of each concentration available to use.
(a) (i) Complete Fig. 1.3 to show how you will prepare your serial dilution.
Each beaker should have: • a labelled arrow to show the volume of glucose solution transferred • a labelled arrow to show the volume of distilled water, W, added • a label under the beaker to show the concentration of glucose solution. , , 0 cm3 of W , ,
2 M1 is a slide of a stained transverse section through a plant stem.
(a) (i) Draw a large plan diagram of the region on M1 indicated by the shaded area in Fig. 2.1. Use a sharp pencil.
Use one ruled label line and label to identify the xylem. draw this region Fig. 2.1 [5] , ,
(ii) Observe the xylem vessel elements in the stem on M1.
Select a group of four adjacent xylem vessel elements.
Each xylem vessel element must touch at least one other xylem vessel element. • Make a large drawing of this group of four xylem vessel elements. • Use one ruled label line and label to identify the wall of one xylem vessel element. [5] , ,
(b) Fig. 2.2 is a photomicrograph of a stained transverse section of a stem from a different plant to M1. Fig. 2.2
Identify three observable differences, other than colour, between the stem section on M1 and the stem section in Fig. 2.2.
Record these three observable differences in an appropriate table. [4] , ,
(c) Fig. 2.3 shows a photomicrograph of a stage micrometer scale that is being used to calibrate an eyepiece graticule.
One division, on either the stage micrometer scale or the eyepiece graticule, is the distance between two adjacent lines.
The length of one division on the stage micrometer in Fig. 2.3 is 1.0 mm. stage micrometer eyepiece graticule 50 60 70 80 90 0 10 20 30 40 100 Fig. 2.3
(i) Calculate the actual length of one eyepiece graticule unit shown in Fig. 2.3.
Give your answer in micrometres (μm).
Show your working and give your answer to three significant figures.
actual length of one eyepiece graticule unit = μm [3] , ,
(ii) Fig. 2.4 is the same photomicrograph as that shown in Fig. 2.2. This was taken with the same microscope and the same lenses used to take the photomicrograph in Fig. 2.3.
The eyepiece graticule has been placed across the length of a vascular bundle. 50 60 70 80 90 0 10 20 30 40 100 Fig. 2.4
Use the calibration of the eyepiece graticule unit from (c)(i) to calculate the actual length of the vascular bundle in Fig. 2.4.
Show your working and use appropriate units.
actual length of the vascular bundle = [2] [Total: 19] , ,
(a) (i) Draw a large plan diagram of the region on M1 indicated by the shaded area in Fig. 2.1. Use a sharp pencil. Use one ruled label line and label to identify the xylem. draw this region Fig. 2.1 [5] , ,
(ii) Observe the xylem vessel elements in the stem on M1. Select a group of four adjacent xylem vessel elements. Each xylem vessel element must touch at least one other xylem vessel element. • Make a large drawing of this group of four xylem vessel elements. • Use one ruled label line and label to identify the wall of one xylem vessel element. [5] , ,
(b) Fig. 2.2 is a photomicrograph of a stained transverse section of a stem from a different plant to M1. Fig. 2.2 Identify three observable differences, other than colour, between the stem section on M1 and the stem section in Fig. 2.2. Record these three observable differences in an appropriate table. [4] , ,
(c) Fig. 2.3 shows a photomicrograph of a stage micrometer scale that is being used to calibrate an eyepiece graticule. One division, on either the stage micrometer scale or the eyepiece graticule, is the distance between two adjacent lines. The length of one division on the stage micrometer in Fig. 2.3 is 1.0 mm. stage micrometer 0 10 20 30 40 100 50 60 70 80 90 eyepiece graticule Fig. 2.3 (i) Calculate the actual length of one eyepiece graticule unit shown in Fig. 2.3. Give your answer in micrometres (μm). Show your working and give your answer to three significant figures. actual length of one eyepiece graticule unit = ......................................................... μm [3] , ,
(ii) Fig. 2.4 is the same photomicrograph as that shown in Fig. 2.2. This was taken with the same microscope and the same lenses used to take the photomicrograph in Fig. 2.3. The eyepiece graticule has been placed across the length of a vascular bundle. 0 10 20 30 40 50 60 70 80 90 100 Fig. 2.4 Use the calibration of the eyepiece graticule unit from (c)(i) to calculate the actual length of the vascular bundle in Fig. 2.4. Show your working and use appropriate units. actual length of the vascular bundle = ............................................................... [2] [Total: 19]